解剖学报

›› 2011, Vol. 42 ›› Issue (5): 622-629.doi: 10.3969/j.issn.0529-1356.2011.05.010

• 细胞和分子生物学 • 上一篇    下一篇

干扰素-γ诱导大鼠肠黏膜微血管内皮细胞表达细胞黏附因子-1和干扰素-γ受体α

薛久洲1,2;崔永勇1;张涛2;刘树强3;段慧琴1;杨佐君1;胡格1*;穆祥1,2*   

  1. 1.北京农学院动物科学技术学院，北京102206; 2.兽医学（中医药）北京市重点实验室，北京102206; 3.北京林业大学生物科技学院，北京100083
  • 收稿日期:2011-02-18 修回日期:2011-03-30 出版日期:2011-10-06
  • 通讯作者: 胡格;穆祥

IFN-γ inducing ICAM-1 and IFN-γRα expression in cultured rat intestinal mucosa microvascular endothelial cells

  1. 1.Animal Science and Technology Department, Beijing University of Agriculture, Beijing102206, China; 2.Bei jing Key Laboratory of Traditional Chinese Veterinary Medicion, Beijing102206, China;3.College of Biological Science and Technology, Beijing Forestry University, Beijing100083, China
  • Received:2011-02-18 Revised:2011-03-30 Online:2011-10-06
  • Contact: HU Ge; MU Xiang

关键词: 细胞黏附因子-1, 干扰素-γ, 干扰素-γ受体α, 微血管内皮细胞, 流式细胞术, 大鼠

Abstract: Objective To investigate the influences of interferon-γ(IFN-γ) on the intercellular cell adhesion molecule-1(ICAM-1) and IFN-γRα expression in the cultured rat intestinal mucosa microvascular endothelial cells (RIMMVECs). Methods After the RIMMVECs separation culture, IFN-γ in different doses were used to induce the EM>in vitro/EM> cultured RIMMVECs, ICAM-1 and IFN-γRα expression in the cultured RIMMVECs at both the mRNA and protein levels which were detected by realtime PCR and flow cytometry, respectively. Results The results showed that RIMMVECs were activated by IFN-γ at the concentrations of 20μg/L and 40 μg/L. ICAM-1 expression increased in the activated RIMMVECs at both mRNA and protein levels, reaching peak effects at 6 hours; IFN-γRα mRNA expression was increased, but the protein decreased after stimulation by 20 μg/L; IFN-γRα mRNA expression peaked at 2 hours and then decreased after 6 hours when stimulated by

Key words: Intercellular cell adhesion-1, Interferon-γ, Interferon-γ receptor-α, Microvascular endothelial cell, Flow cytometry, Rat

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